Shifting the Hydrolysis Equilibrium of Substrate Loaded Acyl Carrier Proteins
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چکیده
منابع مشابه
Expression and purification of four different rhizobial acyl carrier proteins.
In rhizobia, besides the constitutive acyl carrier protein (AcpP) involved in the biosynthesis and transfer of common fatty acids, there are at least three specialized acyl carrier proteins (ACPs): (1) the flavonoid-inducible nodulation protein NodF; (2) the RkpF protein, which is required for the biosynthesis of rhizobial capsular polysaccharides; and (3) AcpXL, which transfers 27-hydroxyoctac...
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The acyl carrier protein (ACP) requires posttranslational modification with a 4'-phosphopantetheine arm for activity, and this thiol-terminated modification carries cargo between enzymes in ACP-dependent metabolic pathways. We show that acyl-ACP synthetases (AasSs) from different organisms are able to load even, odd, and unnatural fatty acids onto E. coli ACP in vitro. Vibrio harveyi AasS not o...
متن کاملSpinach acyl-carrier proteins: structure, regulation, and progress towards cloning.
specificity is determined by the condensing enzyme, namely 8-ketoacyl-[acyl-carrier-proteinlsynthase 11. For the barley complex they are CoA derivatives which give rise to the C,,, C,, and C,, 3-oxoacyl key intermediates. One can speculate that the condensing enzyme activity specified by the cer-cqu-determined polypeptide is responsible for the noted substrate specificity. Therefore, should one...
متن کاملA determinant of substrate specificity predicted from the acyl-acyl carrier protein desaturase of developing cat's claw seed.
Cat's claw (Doxantha unguis-cati L.) vine accumulates nearly 80% palmitoleic acid (16:1Delta9) plus cis-vaccenic acid (18:1Delta11) in its seed oil. To characterize the biosynthetic origin of these unusual fatty acids, cDNAs for acyl-acyl carrier protein (acyl-ACP) desaturases were isolated from developing cat's claw seeds. The predominant acyl-ACP desaturase cDNA identified encoded a polypepti...
متن کاملStructure of the enzyme-acyl carrier protein (ACP) substrate gatekeeper complex required for biotin synthesis.
Although the pimeloyl moiety was long known to be a biotin precursor, the mechanism of assembly of this C7 α,ω-dicarboxylic acid was only recently elucidated. In Escherichia coli, pimelate is made by bypassing the strict specificity of the fatty acid synthetic pathway. BioC methylates the free carboxyl of a malonyl thioester, which replaces the usual acetyl thioester primer. This atypical prime...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2019
ISSN: 0006-2960,1520-4995
DOI: 10.1021/acs.biochem.9b00612